![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4349001:Design
Designed by: Vanessa Pasa Group: iGEM22_UFMG_UFV_Brazil (2022-08-02)
CfcI exochitinase from Aspergillus niger
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 518
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 105
Design Notes
The team UFMG_UFV_Brazil tested if the sequence obtained from the literature had any restriction sites that would compromise BioBrick Assembly, and we found one PstI site. We optimized the problematic codon and changed its sequence to maintain the expression of the aminoacid cysteine.
Source
https://www.ncbi.nlm.nih.gov/nuccore/XM_001400452.2?report=fasta
References
van Munster, J. M., van der Kaaij, R. M., Dijkhuizen, L., & van der Maarel, M. J. E. C. (2012). Biochemical characterization of Aspergillus niger CfcI, a glycoside hydrolase family 18 chitinase that releases monomers during substrate hydrolysis. Microbiology, 158(8), 2168–2179. https://doi.org/10.1099/mic.0.054650-0